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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
CO2 and lactate
CO2 and NH3
Lactate and ammonium

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
increase in specific lactate production from glucose
both (a) and (b)
incomplete glutamine oxidation

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
They act as growth inhibitors
Lactate helps in the growth while ammonium inhibits the growth
Have no effect on cells

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

both (a) and (b)
altering pH of the medium
inhibiting respiration
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Lactic acid
Creatine phosphate
Glucose

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
All of these
Cytokines
Glucose and Glutamine

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