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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture
All of these

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
presence of CO2
addition of bases
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these
DNA degradation

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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