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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
1-2 mmol/litre
7-15 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glucose concentration is required
low glutamine concentration is required
high glutamine concentration is required
high glucose concentration is required

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
All of these
Growth factors
Cytokines

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
enzymatic digestion
All of these
physical disruption

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
It participates in the de novo synthesis of purines and pyrimidines
All of these
Helps in the cells respiration

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