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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
7-15 mmol/litre
1-2 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
oxidative pathway
anaerobic pathway
both (a) and (b)

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
CO2 and lactate
Lactate and ammonium
Lactate only

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
low glucose concentration is required
high glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
none of these
presence of bicarbonate buffer
presence of CO2

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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