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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
anaerobic pathway
both (a) and (b)
oxidative pathway

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
established cell lines
secondary cell lines
propagated cell lines

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

reduction in the pH of culture hence loss of cell viability
increase in pH
no loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

all of these
Down's syndrome
Turner's syndrome
Klinefelter's syndrome

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre
2-7 mmol/litre

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