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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
none of these
both (a) and (b)
oxidative pathway

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
enzymatic digestion
treating with chelating agents
physical disruption

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture
Helps in the cells respiration

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Glucose
ATP
Lactic acid
Creatine phosphate

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
280-360
360-400
150-300

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
CO2 and lactate
Lactate only

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