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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
oxidative pathway
anaerobic pathway
none of these

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
6 amino acids
12 amino acids

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
physical disruption
All of these
enzymatic digestion

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
none of these
addition of bases
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
both (a) and (b)
increase in specific lactate production from glucose
incomplete glutamine oxidation

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
All of these
For monitoring pH of the culture
It participates in the de novo synthesis of purines and pyrimidines

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