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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
presence of CO2
presence of bicarbonate buffer
addition of bases

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

uptake of new genetic material
both (a) and (b)
release of genetic information
phenotypic modifications of cells in culture

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
They act as growth promoters

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glucose concentration is required
low glutamine concentration is required
high glutamine concentration is required
high glucose concentration is required

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
anaerobic pathway
both (a) and (b)
none of these

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

all of these
Down's syndrome
Klinefelter's syndrome
Turner's syndrome

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