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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
presence of bicarbonate buffer
none of these
presence of CO2

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it is too opaque

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
2-5%
1-10%
15-20%

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by substituting glutamine by glutamate
All of these
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Carrel flask culture
Roller test tube culture
Slide culture

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on agar
Organ cultures on plasma clots
All of these

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