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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
presence of bicarbonate buffer
none of these
addition of bases

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Carrel flask culture
Roller test tube culture
Slide culture

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
stable primary cell lines
established cell lines
cell transformations

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
low glucose concentration is required
high glucose concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
Helps in the cells respiration
All of these
It participates in the de novo synthesis of purines and pyrimidines

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