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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on agar
All of these
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

reduction in the pH of culture hence loss of cell viability
no change in pH
no loss of cell viability
increase in pH

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these
DNA degradation

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
oxidative pathway
both (a) and (b)
anaerobic pathway

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
Lactate and ammonium
CO2 and lactate
CO2 and NH3

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
treating with chelating agents
All of these

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