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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
Whole embryo cultures
All of these
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
low glucose concentration is required
low glutamine concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
15 - 20 mmol/litre
7-15 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
6 amino acids
12 amino acids
8 amino acids

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
anaerobic pathway
oxidative pathway
none of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
Lactate only
Lactate and ammonium
CO2 and lactate

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