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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
cell transformations
established cell lines
stable primary cell lines

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
enzymatic digestion
All of these

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
established cell lines
propagated cell lines
secondary cell lines

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