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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
15-20%
2-5%
1-10%

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it is too opaque
it doesn't produce solid clots
it forms a clear and solid coagulum even after dilution
it forms a semi solid coagulum

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
unstable
anchorage independent
stable

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
They act as growth inhibitors
Lactate helps in the growth while ammonium inhibits the growth
Have no effect on cells

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
6 amino acids
10 amino acids
8 amino acids

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