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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
anchorage independent
stable
unstable

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
All of these
lipid peroxidation

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
5.5 - 55 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
stable primary cell lines
none of these
cell transformations

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
1-2 mmol/litre
15 - 20 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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