Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
Helps in the cells respiration
All of these
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

50 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
40 times at an interval of 3 days between subcultures

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

inhibiting the cells from their supporting matrix
None of these
releasing the cells from their supporting matrix
disintegrating the cells from their supporting matrix

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