Animal Cell Culture and Regulation Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 150-300 360-400 300-325 280-360 150-300 360-400 300-325 280-360 ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? They act as growth promoters Have no effect on cells They act as growth inhibitors Lactate helps in the growth while ammonium inhibits the growth They act as growth promoters Have no effect on cells They act as growth inhibitors Lactate helps in the growth while ammonium inhibits the growth ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What are the main constituents of culture for animal cell growth? Cytokines Growth factors All of these Glucose and Glutamine Cytokines Growth factors All of these Glucose and Glutamine ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Accumulation of lactate leads to increase in pH no loss of cell viability reduction in the pH of culture hence loss of cell viability no change in pH increase in pH no loss of cell viability reduction in the pH of culture hence loss of cell viability no change in pH ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? Glycolysis is being inhibited Ethyl alcohol is being produced in excess The cells have too much oxygen The cells do not have enough oxygen Glycolysis is being inhibited Ethyl alcohol is being produced in excess The cells have too much oxygen The cells do not have enough oxygen ANSWER DOWNLOAD EXAMIANS APP