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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
150-300
300-325

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
All of these
Organ cultures on plasma clots
Whole embryo cultures

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
release of genetic information
both (a) and (b)
uptake of new genetic material

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
anaerobic pathway
oxidative pathway
both (a) and (b)

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
2-5%
1-10%
15-20%

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
They act as growth inhibitors
Have no effect on cells

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