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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
300-325
360-400
150-300

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
established cell lines
none of these
stable primary cell lines

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

55 - 75 mmol/litre
105-150 mmol/litre
5.5 - 55 mmol/litre
75-105 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Slide culture
Adherent primary culture
Carrel flask culture

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it is too opaque
it forms a clear and solid coagulum even after dilution
it forms a semi solid coagulum

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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