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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
All of these
Glucose and Glutamine
Cytokines

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Adherent primary culture
Carrel flask culture
Slide culture

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
increase in specific lactate production from glucose
accumulation of ammonia
incomplete glutamine oxidation

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
10-15%
2-5%
15-20%

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