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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Growth factors
Cytokines
Glucose and Glutamine

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
treating with chelating agents
All of these

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
none of these
presence of bicarbonate buffer
addition of bases

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a clear and solid coagulum even after dilution
it forms a semi solid coagulum
it is too opaque
it doesn't produce solid clots

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