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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
Cytokines
All of these
Growth factors

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
For monitoring pH of the culture
All of these

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
primary cell lines
established cell lines
propagated cell lines

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
both (a) and (b)
incomplete glutamine oxidation
accumulation of ammonia

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
Have no effect on cells
They act as growth promoters

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
5.5 - 55 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre

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