Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
propagated cell lines
primary cell lines
established cell lines

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

50 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures

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