Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
All of these
lipid peroxidation

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

40 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

Glycolysis is being inhibited
The cells have too much oxygen
Ethyl alcohol is being produced in excess
The cells do not have enough oxygen

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