Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is correct in terms of determination of location of genetic traits? Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes None of these Restriction sites allow DNAs to be digested Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes None of these Restriction sites allow DNAs to be digested ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes All of these cut DNAs producing a blunt end protect bacteria from viral infection cut DNA in a staggered fashion All of these cut DNAs producing a blunt end protect bacteria from viral infection cut DNA in a staggered fashion ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid All of these always contains an origin of replication usually contains one or more restriction sites is a circular DNA molecule All of these always contains an origin of replication usually contains one or more restriction sites is a circular DNA molecule ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is not true of small inbreeding populations? Alleles may become fixed There is an increased incidence of recessive diseases They tend to loose genetic diversity Mutation is increased Alleles may become fixed There is an increased incidence of recessive diseases They tend to loose genetic diversity Mutation is increased ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because both techniques rely on a constant charge to mass ratio All of these in both cases molecules migrate to the anode both techniques utilize the sieving properties of gels both techniques rely on a constant charge to mass ratio All of these in both cases molecules migrate to the anode both techniques utilize the sieving properties of gels ANSWER DOWNLOAD EXAMIANS APP
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