Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Sau 3A & Bam HI Eco RI & MspI Mbo I & Sau 3A Msp I & HPA II Sau 3A & Bam HI Eco RI & MspI Mbo I & Sau 3A Msp I & HPA II ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Eastern blot Southern blot Western blot Northern blot Eastern blot Southern blot Western blot Northern blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host always contains DNA segments for the regulation of mRNA production All of these always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host always contains DNA segments for the regulation of mRNA production All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these restriction enzymes produce blunt ends? SaII XhoI EcoRV HindIII SaII XhoI EcoRV HindIII ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type IV Type I Type III Type II Type IV Type I Type III Type II ANSWER DOWNLOAD EXAMIANS APP
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