Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends restriction fragments ligases single strands sticky ends restriction fragments ligases single strands ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The TP53 gene of chromosome 17 codes for a protein __________ . that plays a role in the digestive process that is like a white blood cell protein involved in the regulation of the cell cycle involved in glucose transport that plays a role in the digestive process that is like a white blood cell protein involved in the regulation of the cell cycle involved in glucose transport ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid always contains an origin of replication is a circular DNA molecule All of these usually contains one or more restriction sites always contains an origin of replication is a circular DNA molecule All of these usually contains one or more restriction sites ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Why is golden rice pale yellow in color? It is rich in beta-carotene. It is rich in chlorophyll b. It is rich in phycobilins. It is rich in chlorophyll a. It is rich in beta-carotene. It is rich in chlorophyll b. It is rich in phycobilins. It is rich in chlorophyll a. ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is All of these different length fragments of DNA resulting from loss or gain of a restriction site an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved All of these different length fragments of DNA resulting from loss or gain of a restriction site an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved ANSWER DOWNLOAD EXAMIANS APP
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