Recombinant DNA Technology
An expression vector

usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production
always contains an origin of replication
All of these

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Recombinant DNA Technology
A human cDNA library

cannot be used to obtain human genes because it would be radioactive
None of these
contains DNA for virtually all of the human proteins in vectors
contains DNA for specific human proteins

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Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence

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