Recombinant DNA Technology
A human cDNA library

cannot be used to obtain human genes because it would be radioactive
contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors
None of these

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
All of these
always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host

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Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene

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