Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
All of these
always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
None of these
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors

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