Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

none of these
pRK 2013
pRN 3
either (a) or (b)

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

two sets of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

intact cells are used
All of these
gene can be transferred to many cells simultaneously
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

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