Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

either (a) or (b)
pRK 2013
none of these
pRN 3

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Gene Transfer in Plants
Which of the following is true about T DNA?

Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
Integration of T DNA occurs at two specific sites in the plant nuclear DNA

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Gene Transfer in Plants
In a plant tumour cell

only a small specific segment of callus T DNA is incorporated
may vary from plant to plant
different parts of the Ti-plasmid are incorporated
complete Ti plasmid is incorporated in plant nuclear DNA

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

one set of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
two sets of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer

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