Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRK 2013
all of these
pRN 3
pBR 322 based plasmid vector

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

All of these
gene can be transferred to many cells simultaneously
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
intact cells are used

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