Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRN 3
all of these
pRK 2013
pBR 322 based plasmid vector

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Gene Transfer in Plants
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

It employs needle with diameter greater than cell diameter
All of these
It is carried out with the help of micromanipulator
The recipient cells are immobilized on artificial support or artificially bound to substarate

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Gene Transfer in Plants
In a plant tumour cell

complete Ti plasmid is incorporated in plant nuclear DNA
only a small specific segment of callus T DNA is incorporated
different parts of the Ti-plasmid are incorporated
may vary from plant to plant

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