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Gene Transfer in Plants

Gene Transfer in Plants
Which technique is used to introduce genes into dicots?

electroporation
microinjection
Ti plasmid infection
particle acceleration

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Gene Transfer in Plants
Microinjection involves

injection of DNA into bigger cells
injection with needle having diameter greater than cell diameter
injection of large amount of DNA
All of these

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Gene Transfer in Plants
Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?

vir A and vir B
vir A and vir G
vir C and vir D
vir B and vir E

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRK 2013
all of these
pBR 322 based plasmid vector
pRN 3

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

either (a) or (b)
pRK 2013
none of these
pRN 3

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

>235 kb
140-235 kb
40-80 kb
80-120 kb

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