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Gene Transfer in Plants

Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

polyethylene glycol (PEG)
decanal
luciferin
all of these

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Gene Transfer in Plants
In the liposome mediated gene transfer in plants, nucleic acids are

protected from nuclease digestion
not stable in liposomes
stable in liposomes
both (a) and (b)

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Gene Transfer in Plants
Integrated nopaline T-DNA occurs as

two segments
four segments
three segments
single segment

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Gene Transfer in Plants
Plant transformation vectors based on Agrobacterium can generally be divided into

eight vectors
two vectors
six vectors
four vectors

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

>235 kb
40-80 kb
80-120 kb
140-235 kb

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

These are important in integration mechanism
They are conserved between nopaline and octopine Ti-plasmids
All of these
These repeats are transferred intact to the plant genome

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