Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

two sets of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

gene can be transferred to many cells simultaneously
All of these
intact cells are used
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

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Gene Transfer in Plants
Which of the following is true about T DNA?

Integration of T DNA occurs at two specific sites in the plant nuclear DNA
Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

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