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Gene Transfer in Plants

Gene Transfer in Plants
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

12 bp
30 bp
20 bp
25 bp

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRN 3
all of these
pRK 2013
pBR 322 based plasmid vector

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Gene Transfer in Plants
Integrated nopaline T-DNA occurs as

three segments
two segments
four segments
single segment

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

These repeats are transferred intact to the plant genome
These are important in integration mechanism
They are conserved between nopaline and octopine Ti-plasmids
All of these

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Gene Transfer in Plants
The left segment of octopine T-DNA (TL) is necessary for

tumour formation
conjugative transfer
all of these
enzymes for agropine biosynthesis

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Gene Transfer in Plants
Which technique is used to introduce genes into dicots?

particle acceleration
electroporation
Ti plasmid infection
microinjection

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