Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ have identical DNA sequences be of the same length originate from the same type of cell be cut by the same restriction enzyme have identical DNA sequences be of the same length originate from the same type of cell be cut by the same restriction enzyme ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site All of these cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site All of these cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes cut DNA in a staggered fashion cut DNAs producing a blunt end protect bacteria from viral infection All of these cut DNA in a staggered fashion cut DNAs producing a blunt end protect bacteria from viral infection All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. negative ... positive long ... short positive to negative basic ... acidic negative ... positive long ... short positive to negative basic ... acidic ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology First discovered, Type II restriction endonuclease was EcoRI Hind II Eco K Hinf I EcoRI Hind II Eco K Hinf I ANSWER DOWNLOAD EXAMIANS APP
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