Recombinant DNA Technology
In order to insert a foreign gene into a plasmid, both must __________

be of the same length
be cut by the same restriction enzyme
have identical DNA sequences
originate from the same type of cell

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Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition site and different recognition sequence
same recognition site and recognition sequence
same recognition sequence but different recognition site

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence

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