Recombinant DNA Technology
When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .

assortative mating
heterosis
inbreeding
genetic drift

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Recombinant DNA Technology
Isoschizomers recognize

same recognition site and recognition sequence
different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and different recognition sequence

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains an origin of replication

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence

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