Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

50 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

The cells have too much oxygen
Glycolysis is being inhibited
The cells do not have enough oxygen
Ethyl alcohol is being produced in excess

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