Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
incomplete glutamine oxidation
increase in specific lactate production from glucose
both (a) and (b)

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

disintegrating the cells from their supporting matrix
None of these
inhibiting the cells from their supporting matrix
releasing the cells from their supporting matrix

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

The cells have too much oxygen
Ethyl alcohol is being produced in excess
Glycolysis is being inhibited
The cells do not have enough oxygen

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