Cloning Vectors Charon vectors are different from EMBL vectors because they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is required physical separation of lambda arm from central fragment is not required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is required physical separation of lambda arm from central fragment is not required both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 20-25 kb 25-30 kb 40-50 kb 18-20 kb 20-25 kb 25-30 kb 40-50 kb 18-20 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain λ att site Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain λ att site Contain functional origin of replication of the plasmid and λ phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Difference between λ gt 10 and λ gt 11 vectors is that λ gt 10 is an expression vector λ gt 11 is an expression vector λ gt 10 is a replacement vector λ gt 11 is a replacement vector λ gt 10 is an expression vector λ gt 11 is an expression vector λ gt 10 is a replacement vector λ gt 11 is a replacement vector ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmids lack marker genes coding for replication origin of replication genes coding for viral proteins cleavage site for the insertion of foreign DNA marker genes coding for replication origin of replication genes coding for viral proteins cleavage site for the insertion of foreign DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Phagemid vectors are All of these phages carrying properties of plasmids combination of phages and cosmid combination of plasmid and phage λ All of these phages carrying properties of plasmids combination of phages and cosmid combination of plasmid and phage λ ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS