Cloning Vectors EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 40-44 kb 6-7 kb 15-25 kb 1-2 kb 40-44 kb 6-7 kb 15-25 kb 1-2 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 18-20 kb 40-50 kb 20-25 kb 25-30 kb 18-20 kb 40-50 kb 20-25 kb 25-30 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are plasmids that have no selection marker phages that lack cos site cryptic plasmids plasmids that contain fragment of λ DNA including the cos site plasmids that have no selection marker phages that lack cos site cryptic plasmids plasmids that contain fragment of λ DNA including the cos site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain λ att site Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain λ att site Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Charon 34 and Charon 35 can clone DNA upto 6-7 kb 30-35 kb 9-20 kb 1-2 kb 6-7 kb 30-35 kb 9-20 kb 1-2 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmids lack origin of replication marker genes coding for replication genes coding for viral proteins cleavage site for the insertion of foreign DNA origin of replication marker genes coding for replication genes coding for viral proteins cleavage site for the insertion of foreign DNA ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS