Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 20-25 kb 18-20 kb 40-50 kb 25-30 kb 20-25 kb 18-20 kb 40-50 kb 25-30 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Phagemid consist of plasmid vector carrying origin of replication of plasmid only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site plasmid vector carrying origin of replication of λ phage only plasmid vector carrying origin of replication of plasmid only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site plasmid vector carrying origin of replication of λ phage only ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids helps whole genome in circularization and ligation consists of 12 bases both (a) and (b) contains cleavage site helps whole genome in circularization and ligation consists of 12 bases both (a) and (b) contains cleavage site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 40-44 kb 1-2 kb 6-7 kb 15-25 kb 40-44 kb 1-2 kb 6-7 kb 15-25 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Stuffer is the left arm of the vector DNA None of these the right arm of the vector DNA central fragment of the vector DNA the left arm of the vector DNA None of these the right arm of the vector DNA central fragment of the vector DNA ANSWER DOWNLOAD EXAMIANS APP