Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 25-30 kb 20-25 kb 18-20 kb 40-50 kb 25-30 kb 20-25 kb 18-20 kb 40-50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Phagemid consist of plasmid vector carrying λ attachment (λ att) site plasmid vector carrying λ phage's cos site plasmid vector carrying origin of replication of λ phage only plasmid vector carrying origin of replication of plasmid only plasmid vector carrying λ attachment (λ att) site plasmid vector carrying λ phage's cos site plasmid vector carrying origin of replication of λ phage only plasmid vector carrying origin of replication of plasmid only ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are plasmids that have no selection marker phages that lack cos site cryptic plasmids plasmids that contain fragment of λ DNA including the cos site plasmids that have no selection marker phages that lack cos site cryptic plasmids plasmids that contain fragment of λ DNA including the cos site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Conjugative plasmids carry transfer genes called the tra genes do not carry transfer genes exhibit antibiotic resistance do not exhibit antibiotic resistance carry transfer genes called the tra genes do not carry transfer genes exhibit antibiotic resistance do not exhibit antibiotic resistance ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Difference between λ gt 10 and λ gt 11 vectors is that λ gt 11 is a replacement vector λ gt 11 is an expression vector λ gt 10 is an expression vector λ gt 10 is a replacement vector λ gt 11 is a replacement vector λ gt 11 is an expression vector λ gt 10 is an expression vector λ gt 10 is a replacement vector ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS