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Cloning Vectors

Cloning Vectors
Charon 34 and Charon 35 are the examples of

plasmid vector
phage vector
phagemid vector
cosmid vector

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Cloning Vectors
Difference between λ gt 10 and λ gt 11 vectors is that

λ gt 11 is a replacement vector
λ gt 10 is an expression vector
λ gt 10 is a replacement vector
λ gt 11 is an expression vector

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Cloning Vectors
Cos site of the cosmids

consists of 12 bases
both (a) and (b)
helps whole genome in circularization and ligation
contains cleavage site

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Cloning Vectors
Phagemid vectors are

combination of phages and cosmid
phages carrying properties of plasmids
combination of plasmid and phage λ
All of these

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Cloning Vectors
Plasmids which are maintained as multiple copy number per cell are known as

cryptic plasmids
relaxed plasmids
stringent plasmids
none of these

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Cloning Vectors
Cosmid vectors are used for

cloning large fragments of DNA
cloning prokaryotic DNA only
cloning eukaryotic DNA only
cloning small fragments of DNA

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