Cloning Vectors A plasmid can be considered as a suitable cloning vector if it possesses a single restriction site for one or more restriction enzymes All of these insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells it possesses a single restriction site for one or more restriction enzymes All of these insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Select the wrong statement about plasmids? It is double stranded It is extrachromosomal Its replication depends upon host cell It is closed and circular DNA It is double stranded It is extrachromosomal Its replication depends upon host cell It is closed and circular DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Size of the DNA that can be packaged into a λ phage is 50 kb 40-50 kb 35-53 kb any size 50 kb 40-50 kb 35-53 kb any size ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors M 13 is an example of plasmid single stranded DNA vector both (a) and (b) filamentous phage plasmid single stranded DNA vector both (a) and (b) filamentous phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 20-23 kb 40-44 kb 6-7 kb 1-2 kb 20-23 kb 40-44 kb 6-7 kb 1-2 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Phagemid consist of plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site plasmid vector carrying origin of replication of plasmid only plasmid vector carrying origin of replication of λ phage only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site plasmid vector carrying origin of replication of plasmid only plasmid vector carrying origin of replication of λ phage only ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS