Cloning Vectors
Cos site of the cosmids

contains cleavage site
both (a) and (b)
helps whole genome in circularization and ligation
consists of 12 bases

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Cloning Vectors
Conjugative plasmids

do not carry transfer genes
do not exhibit antibiotic resistance
exhibit antibiotic resistance
carry transfer genes called the tra genes

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Cloning Vectors
Cosmid vectors are

phages that lack cos site
plasmids that have no selection marker
cryptic plasmids
plasmids that contain fragment of λ DNA including the cos site

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