Cloning Vectors
Single stranded vectors are useful

for probe preparation
for sequencing of cloned DNA
for oligonucleotide directed mutagenesis
All of these

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Cloning Vectors
Conjugative plasmids

do not exhibit antibiotic resistance
exhibit antibiotic resistance
do not carry transfer genes
carry transfer genes called the tra genes

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Cloning Vectors
Cosmids lack

origin of replication
cleavage site for the insertion of foreign DNA
marker genes coding for replication
genes coding for viral proteins

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