Cloning Vectors
Single stranded vectors are useful

for probe preparation
for sequencing of cloned DNA
All of these
for oligonucleotide directed mutagenesis

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Cloning Vectors
Phagemid consist of

plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying λ phage's cos site
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying origin of replication of plasmid only

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