Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

free protein that is secreted out
β-galactosidase fused protein
free protein in the cytoplasm
All of these

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Cloning Vectors
Cosmids lack

origin of replication
cleavage site for the insertion of foreign DNA
marker genes coding for replication
genes coding for viral proteins

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Cloning Vectors
Conjugative plasmids

carry transfer genes called the tra genes
exhibit antibiotic resistance
do not carry transfer genes
do not exhibit antibiotic resistance

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