Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Protein-coding genes are always associated with a restriction pattern
Restriction sites allow DNAs to be digested
Known protein coding sequences are too far apart to allow linkage determination for most new genes

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