Recombinant DNA Technology Restriction enzymes cut DNAs producing a blunt end protect bacteria from viral infection cut DNA in a staggered fashion All of these cut DNAs producing a blunt end protect bacteria from viral infection cut DNA in a staggered fashion All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. positive to negative long ... short basic ... acidic negative ... positive positive to negative long ... short basic ... acidic negative ... positive ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA primase DNA helicase DNA ligase DNA polymerase DNA primase DNA helicase DNA ligase DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Msp I & HPA II Eco RI & MspI Mbo I & Sau 3A Sau 3A & Bam HI Msp I & HPA II Eco RI & MspI Mbo I & Sau 3A Sau 3A & Bam HI ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
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