Recombinant DNA Technology
Restriction enzymes

cut DNA in a staggered fashion
All of these
cut DNAs producing a blunt end
protect bacteria from viral infection

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains DNA segments for the regulation of mRNA production

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Protein-coding genes are always associated with a restriction pattern
Restriction sites allow DNAs to be digested
Known protein coding sequences are too far apart to allow linkage determination for most new genes
None of these

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