Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and different recognition sequence
same recognition site and recognition sequence

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
always contains an origin of replication
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines

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