Recombinant DNA Technology
A plasmid

usually contains one or more restriction sites
is a circular DNA molecule
always contains an origin of replication
All of these

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
always contains an origin of replication
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
All of these

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