Recombinant DNA Technology
A plasmid

is a circular DNA molecule
always contains an origin of replication
All of these
usually contains one or more restriction sites

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

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Recombinant DNA Technology
Isoschizomers recognize

same recognition site and different recognition sequence
different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and recognition sequence

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