Recombinant DNA Technology
A plasmid

always contains an origin of replication
usually contains one or more restriction sites
is a circular DNA molecule
All of these

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and recognition sequence
same recognition site and different recognition sequence

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Recombinant DNA Technology
A human cDNA library

None of these
cannot be used to obtain human genes because it would be radioactive
contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors

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