Recombinant DNA Technology
A plasmid

All of these
usually contains one or more restriction sites
always contains an origin of replication
is a circular DNA molecule

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus

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