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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Creatine phosphate
ATP
Glucose

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
All of these
For monitoring pH of the culture

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Slide culture
Carrel flask culture
Adherent primary culture

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and lactate
CO2 and NH3
Lactate and ammonium
Lactate only

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
lipid peroxidation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
All of these
enzymatic digestion
physical disruption

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