Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

function as a proton shuttle
neutralize the charge on the other's side chain
keep the specificity pocket open
clamp the substrate into the active site

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

fixing enzymes into membranes so that they are adjacent to each other
concentrating enzymes within specific cellular compartments
All of these
grouping enzymes into free-floating, multienzyme complexes

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A, B, C, and D will all still be produced
A, B, and C will still be produced, but not D
A and B will still be produced, but not C or D
A will still be produced, but not B, C, or D

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
absence of water in the active site
presence of a low-barrier hydrogen bond in the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket

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