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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
both (a) and (b)
semi-solid medium
liquid medium

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
physical disruption
enzymatic digestion
treating with chelating agents

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
secondary cell lines
propagated cell lines
primary cell lines

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
oxidative pathway
anaerobic pathway
both (a) and (b)

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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