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CLONING VECTORS

Cloning Vectors
Cos site of the cosmids

consists of 12 bases
helps whole genome in circularization and ligation
contains cleavage site
both (a) and (b)

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Cloning Vectors
M 13 is an example of

plasmid
filamentous phage
single stranded DNA vector
both (a) and (b)

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Cloning Vectors
Phagemid vectors are

All of these
phages carrying properties of plasmids
combination of plasmid and phage λ
combination of phages and cosmid

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Cloning Vectors
Single stranded vectors are useful

All of these
for oligonucleotide directed mutagenesis
for sequencing of cloned DNA
for probe preparation

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

All of these
free protein that is secreted out
β-galactosidase fused protein
free protein in the cytoplasm

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