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DNA Structure and Replication

DNA Structure and Replication
What is the approximate size (in kb) of the E. coli genome?

5500 kilobase
6500 kilobase
4500 kilobase
3000 kilobase

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DNA Structure and Replication
The nicks or gaps between Okazaki fragments are eventually sealed by

DNA ligase
polymerase
gyrase
RNA ligase

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' endonuclease activity
5' → 3' exonuclease activity
partially 5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity

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DNA Structure and Replication
The DNA is negatively super coiled, complexes to histone,

HU
All of these
HSP-1
H-NS

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DNA Structure and Replication
What is the name given to the points at which a DNA helix is unwound and new strands develop?

Okazaki fragments
Replication forks
Leading strands
Replication origins

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

high temperature and Mg++
low temperature and Ca++
low temperature and Mg++
high temperature and Ca++

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