DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' exonuclease activity
partially 5' → 3' endonuclease activity
5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity

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DNA Structure and Replication
In DNA, there are

five bases known as adenine, guanine, thymine, tryptophan and cytosine
four bases known as adenine, guanine, thymine and cytosine
three bases known as adenine, guanine and cytosine
only two bases known as adenine and cytosine

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DNA Structure and Replication
In a typical electrophoresis experiment, larger fragments of DNA move more

quickly, so they appear nearer the top of a gel photograph
slowly, so they appear nearer the top of a gel photograph
slowly, so they appear nearer the bottom of a gel photograph
quickly, so they appear nearer the bottom of a gel photograph

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)

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