Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
episomally only
either episomally or integrated at low copy number
integrated only

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

not necessary for viral replication
transcriptional regulators and are essential for viral replication
coding for virus coat protein
important for packaging of viral DNA into viral head

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