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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following can be used for reducing the size of gene inserts containing introns?

none of these
Retro virus
Baculo virus
Vaccinia virus

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

pBR 322 vector
COS cell line
SV 40 helper virus
none of these

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

His 3
Trp 1
Ura 3
Lys 2

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

major component of protein matrix in which viruses are embedded
non essential for baculo virus replication
both (a) and (b)
essential for baculo virus replication

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

All of these
Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA
Over production of proteins of commercial value

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

centromere
both (a) and (c)
telomere
autonomously replicating sequence

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