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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

COS cell line
SV 40 helper virus
pBR 322 vector
none of these

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Trp 1
His 3
Lys 2

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Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

centromere
autonomously replicating sequence
telomere
all of these

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Vectors Uses for Animal Cell Culture
The size of the genome of SV 40 is

5.2 kb
110 kb
69 kb
7.2 kb

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Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YEp
Both (a) and (b)
YRp
YCp

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Carries autonomously replicating sequence
Carries centromere and telomere sequences
Has a origin of replication and rep genes
Allows transformation by using crossing over

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