Vectors Uses for Animal Cell Culture
Yeast episomal plasmids (YEp) were constructed using

12μm yeast plasmid and E coli vector pMB 9
12μm yeast plasmid and E coli vector pBR 322
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

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Vectors Uses for Animal Cell Culture
In case of Baculoviruses, the foreign gene

is inserted between the polyhedrin promoter and downstream sequences
is inserted downstream of polyhedrin promoter
is inserted downstream of viral origin of replication
is inserted randomly in viral genome

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Vectors Uses for Animal Cell Culture
Retroviruses have advantage for being used as vector for animal cells because

they cover a wide host range
viral gene expression is driven by strong promoters
infection does not lead to cell death, infected cells produce virus over an indefinite period
All of these

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