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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
established cell lines
stable primary cell lines

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
no change in pH
reduction in the pH of culture hence loss of cell viability
increase in pH

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
For monitoring pH of the culture
Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
anaerobic pathway
none of these
oxidative pathway

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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