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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
cell transformations
none of these
stable primary cell lines

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
both (a) and (b)
uptake of new genetic material
release of genetic information

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
All of these
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

all of these
Klinefelter's syndrome
Turner's syndrome
Down's syndrome

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
presence of CO2
presence of bicarbonate buffer
addition of bases

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
7-15 mmol/litre
1-2 mmol/litre
2-7 mmol/litre

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