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Cloning Vectors

Cloning Vectors
Maximum size of foreign DNA that can be inserted into an insertion vector is

35 kb
27 kb
50 kb
18 kb

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Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Tetr
Ampr
Kanr
Both (a) and (b)

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Cloning Vectors
Single stranded vectors are useful

for sequencing of cloned DNA
for oligonucleotide directed mutagenesis
for probe preparation
All of these

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Cloning Vectors
λ ZAP vector is an example of

phagemid
plasmid
phage
cosmid

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

25-30 kb
40-50 kb
18-20 kb
20-25 kb

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

All of these
β-galactosidase fused protein
free protein in the cytoplasm
free protein that is secreted out

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